Controlling Disease Resistance

Technical Updates

Controlling Disease Resistance

15/02/2014

At this time of year we know only too well that managed turf is often afflicted with disease scars that spoil the appearance and playability of sports turf.

Fortunately we have an armoury of fungicides we can draw on to counter the effects of disease. However, there is a potential risk that some of these products could lose their effectiveness if the target fungi develop a resistance to them. To date there have been no confirmed cases of field resistance to turf fungicides in the UK but this has not been the case in our other agrochemical markets, where developed resistance has seen multi-million pound crop protection products toppled from the market place in the blink of an eye, as farmers and growers claimed compensation for crop losses from the manufacturers.Believe it or not, fungicides have been around for over 200 years. Early fungicides were mainly used in viticulture (grapevines) and as cereal seed treatments. Like caveman’s tools, these products were crude but effective – often quite persistent with a wide spectrum of control. Advances in chemistry have allowed more complex molecules to be synthesised as fungicides and these have tended to be more target specific, which is beneficial to the ecosystem in which they are used. Over the years a major global market has developed with products to control diseases in food crops, ornamentals, forestry, and amenity sports areas. The world market is worth around $8 billion today – so you can see there’s a lot riding on the future of these products and of course the benefits they bring to the table! Stop and think for a moment what life would be like without any fungicides to arrest the spread of disease on sports turf. It could be almost as bad as trying to live without Google!.The Fungicide Resistance Action Group-UK provides advice for combating disease resistance. They recommend the adoption of an integrated approach to controlling fungal disease that includes all aspects of cultural control, use of tolerant grasses, biological and chemical control. In this short article I will examine the important diseases of UK turf and the options available for integrated control but to begin with I shall tackle some of the terminology associated with disease resistance.TerminologyA fungicide will have a spectrum of control that includes some fungal species but not others. Those it controls are said to be ‘sensitive’ to the chemical but all other species have a ‘natural resistance’, which defines the limitations of the product. The type of resistance to chemicals that is developed by a fungus from persistent use is often referred to as ‘acquired resistance’. This is a subtle change in genetics that occurs in the cells of the fungal pathogen, protecting it from the effects of the fungicide. Studies of resistance mechanisms, the processes by which fungi adapt to chemicals, have been carried out to enable the development of new products and strategies that will help to extend the useful life of fungicides for the future.Fungicides work by interfering with natural processes at a cellular level. We refer to this as the fungicide’s ‘biochemical mode of action’. In most cases the mode of action involves blocking a biochemical pathway essential to the growth of the fungus or interfering with the natural regulation of the cell. Some products have only a ‘single site mode of action’ i.e. they disrupt the essential processes at only one point in the cells metabolism, whereas others target multiple sites and are referred to as having a ‘multi-site mode of action’.Pesticides that have similar chemical structures are often classified together, for example the ‘strobilurin’ group contains three active ingredients used in turf; azoxystrobin, pyraclostrobin and trifloxystrobin. These families of chemicals will usually have similar target sites and biochemical modes of action. Therefore, if resistance develops to one of these actives, the others in that group are likely to be tolerated as well. This phenomenon is known as ‘cross resistance’.

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So how does the development of resistance to a chemical occur? If you were to look down a microscope at some Microdochium spores you could be forgiven for saying that they are all the same; they are not.A population of fungal spores consists of many individuals and somewhere amongst them will be one that has a genetic mutation that would allow it to survive the attack of a specific fungicide, by virtue of a minute difference in cellular chemistry. In the absence of this specific fungicide the mutation gives no benefit and so the individuality does not multiply in the population. However, when that community of fungal spores does encounter the fungicide, this one individual benefits by surviving the attack and is able to multiply and pass on this ability to all future generations, so that soon the chemical will have been rendered ineffective. This is a very simplistic illustration of one of the ways that resistance occurs but it is the same process by which all organisms evolve under natural selection.Mechanisms of fungicide resistance that have been discovered include: -The most important turf disease in the UK is Fusarium – now called Microdochium patch (Microdochium nivale). Five other diseases can also occur on a regular basis; Anthracnose, Dollar Spot, Take-all Patch, Red Thread and Leaf Spot.To date, there have been no confirmed cases of disease resistance to any of the current fungicides used in managed amenity turf in the UK, although reduced sensitivity of Michrodochium nivale to iprodione has been observed in a small number of UK isolates in laboratory assays. Resistance to some fungicides used against Michrodochium Patch, Anthracnose and Dollar Spot has been demonstrated elsewhere in Europe and the USA.Integrated ControlWith heavily used turf surfaces it is difficult to avoid fungicide applications completely but if disease is becoming an all too regular occurrence on your grass, then you may need to re-examine and make changes to the maintenance programme. Cultural factors that encourage the outbreak of disease can be summarised (by disease) as follows:Michrodochium patchAnthracnoseDollar SpotTake-all Patch – usually only found on newly established turfRed Thread – This disease can usually be managed without fungicidesLeaf SpotReferences (All available online)1. Keith J Brent and Derek W Hollomon (2007) Fungicide Resistance in Crop Pathogens: How can it be managed? 2nd Edition published by the Fungicide Resistance Action Committee (Search: fungicide resistance in crop uk)2. Anon: Fungicide Resistance Management in Turfgrass – Leaflet published by the Fungicide Resistance Action Committee (Search: frac turf) This article is now closed for BASIS point allocation.